Rat Epcr / PROCR Protein (His tag) – PKSR030205

Recombinant Rat Epcr / PROCR Protein (His tag)

Endothelial protein C receptor (EPCR), also known as activated protein C receptor (APC receptor) or PROCR, is a receptor for Protein C. Protein C plays an important role in many metabolism processes in humans and other animals after activated by binding to Endothelial protein C receptor (EPCR). Because of the EPCR is found primarily on endothelial cells (cells on the inside of blood vessels), activated protein C is found maily near endothelial cells. Protein C is pleiotropic, with two main functions: anticoagulation and cytoprotection. Which function will be performed depend on whether or not protein C remains bind to EPCR after activated. The anticoagulation occurs when it does not. In this case, protein C functions as an anticoagulant by irreversibly proteolytically inactivating Factor Va and Factor VIIIa, turning them into Factor Vi and Factor VIIIi respectively. When still bound to EPCR, activated protein C performs its cytoprotective effects, acting on the effector substrate PAR-1, protease-activated receptor-1. To a degree, APC’s anticoagulant properties are independent of its cytoprotective ones, in that expression of one pathway is not affected by the existence of the other.

Synonym(s) : PROCR

Species(s) : Rat

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the rat PROCR (Met1-Ser213) was expressed with a polyhistidine tag at the C-terminus.

Predicted N-terminal amino acid : Asn 21

Molecular weight/mass : The recombinant rat PROCR comprises 204 amino acids and predicts a molecular mass of 23.2 kDa. The apparent molecular mass of the recombinant protein is approximately 33-37 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Protein Construction : A DNA sequence encoding the rat PROCR (Met1-Ser213) was expressed with a polyhistidine tag at the C-terminus.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 95 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet