Rat ANP (Atrial Natriuretic Peptide) ELISA Kit – E-EL-R0017

Rat ANP (Atrial Natriuretic Peptide) ELISA Kit

The ANP ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with ANP. During the reaction, Rat ANP in the sample or standard competes with a fixed amount of ANP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat ANP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat ANP in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Rat Atrial Natriuretic Peptide

Abbreviation : ANP

Uniprot ID :

Format : 96 rx

Reactivity : Rat

ELISA configuration : Competitive

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 – 1000 pg/ml

Manual & Datasheet

More information about this Rat ANP (Atrial Natriuretic Peptide) ELISA Kit from Elabscience