Rat ANG2 (Angiopoietin 2) ELISA Kit – E-EL-R0125

Rat ANG2 (Angiopoietin 2) ELISA Kit

The ANG2 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ANG2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ANG2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ANG2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ANG2. You can calculate the concentration of ANG2 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Angiopoietin 2

Abbreviation : ANG2

Uniprot ID : O35462

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 37.5 pg/ml

Detection Range : 62.5 – 4000 pg/ml

Manual & Datasheet

More information about this Rat ANG2 (Angiopoietin 2) ELISA Kit from Elabscience