Rat AMY1 (Amylase Alpha 1, Salivary) ELISA Kit – E-EL-R2544

Rat AMY1 (Amylase Alpha 1, Salivary) ELISA Kit

The AMY1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat AMY1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat AMY1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AMY1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat AMY1. You can calculate the concentration of Rat AMY1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Amylase Alpha 1, Salivary

Abbreviation : AMY1

Uniprot ID : Q7M051

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.938 ng/ml

Detection Range : 1.563 – 100 ng/ml

Manual & Datasheet

More information about this Rat AMY1 (Amylase Alpha 1, Salivary) ELISA Kit from Elabscience