Rat ADH (Antidiuretic Hormone) ELISA Kit – E-EL-R0522

Rat ADH (Antidiuretic Hormone) ELISA Kit

The ADH ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with ADH/VP/AVP. During the reaction, Rat ADH/VP/AVP in the sample or standard competes with a fixed amount of ADH/VP/AVP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat ADH/VP/AVP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat ADH/VP/AVP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Detected Target name: Rat Antidiuretic Hormone

Abbreviation : ADH

Uniprot ID :

Format : 96 rx

Reactivity : Rat

ELISA configuration : Competitive

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 – 1000 pg/ml

Manual & Datasheet

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