Mouse U-T4 (Ultrasensitivity Thyroxine) ELISA Kit – E-EL-M1221

Mouse U-T4 (Ultrasensitivity Thyroxine) ELISA Kit

The Mouse u-T4 ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with u-T4. During the reaction, Mouse u-T4 in the sample or standard competes with a fixed amount of u-T4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse u-T4. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse u-T4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Mouse Ultrasensitivity Thyroxine

Abbreviation : U-T4

Uniprot ID :

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Competitive

Sensitivity : 0.844 pg/ml

Detection Range : 1.406 – 90 pg/ml

Manual & Datasheet

More information about this Mouse U-T4 (Ultrasensitivity Thyroxine) ELISA Kit from Elabscience