Mouse TSLP (Thymic Stromal Lymphopoietin) ELISA Kit – E-EL-M0646

Mouse TSLP (Thymic Stromal Lymphopoietin) ELISA Kit

The Mouse TSLP ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TSLP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TSLP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TSLP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TSLP. You can calculate the concentration of TSLP in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse Thymic Stromal Lymphopoietin

Abbreviation : TSLP

Uniprot ID : Q969D9

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 – 1000 pg/ml

Manual & Datasheet

More information about this Mouse TSLP (Thymic Stromal Lymphopoietin) ELISA Kit from Elabscience