Mouse TSAb (Thyroid Stimulating Hormone Antibody) ELISA Kit
The Mouse TSAb ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antigen specific to Mouse TSAb. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antigen. Then a biotinylated detection antigen specific for Mouse TSAb and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TSAb, biotinylated detection antigen and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TSAb. You can calculate the concentration of Mouse TSAb in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Mouse Anti-Thyroid Stimulating Hormone
Abbreviation : TSH-Ab
Format : 96 rx
Reactivity : Mouse
ELISA configuration : Sandwich
Sensitivity : 1.875 ng/ml
Detection Range : 3.125 – 200 ng/ml
Additional information
Format | 96 rx |
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Reactivity | Mouse |
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