Mouse Thrombopoietin antibody ELISA Kit – E-EL-M1141

Mouse Thrombopoietin antibody ELISA Kit

The ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antigen specific to Mouse Thrombopoietin antibody. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antigen. Then a biotinylated detection antigen specific for Mouse Thrombopoietin antibody and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Thrombopoietin antibody, biotinylated detection antigen and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Thrombopoietin antibody. You can calculate the concentration of Mouse Thrombopoietin antibody in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse Anti-Thrombopoietin

Abbreviation : TPO-Ab

Uniprot ID : 

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 1.875 ng/ml

Detection Range : 3.125 – 200 ng/ml

Manual & Datasheet

More information about this Mouse Thrombopoietin antibody ELISA Kit from Elabscience