Mouse BMP-4 (Bone Morphogenetic Protein 4) ELISA Kit – E-EL-M0194

Mouse BMP-4 (Bone Morphogenetic Protein 4) ELISA Kit

The Mouse BMP-4 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BMP-4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for BMP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BMP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of BMP-4. You can calculate the concentration of BMP-4 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse Bone Morphogenetic Protein 4

Abbreviation : BMP-4

Uniprot ID : P21275

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 18.75 pg/ml

Detection Range : 31.25 – 2000 pg/ml

Manual & Datasheet

More information about this Mouse BMP-4 (Bone Morphogenetic Protein 4) ELISA Kit from Elabscience