Mouse AgRP (Agouti Related Protein) ELISA Kit
The Mouse AgRP ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AgRP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for AgRP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AgRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of AgRP. You can calculate the concentration of AgRP in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Mouse Agouti Related Protein
Abbreviation : AgRP
Uniprot ID : P56473
Format : 96 rx
Reactivity : Mouse
ELISA configuration : Sandwich
Sensitivity : 9.375 pg/ml
Detection Range : 15.625 – 1000 pg/ml
More information about this Mouse AgRP (Agouti Related Protein) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
---|---|
Reactivity | Mouse |
Ask a question about Mouse AgRP (Agouti Related Protein) ELISA Kit – E-EL-M1356
You must be logged in to post a review.
Reviews