Mouse α2-AP (α2-Antiplasmin) ELISA Kit – E-EL-M1252

Mouse α2-AP (α2-Antiplasmin) ELISA Kit

The Mouse α2-AP ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse α2-AP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse α2-AP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse α2-AP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse α2-AP. You can calculate the concentration of Mouse α2-AP in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse α2-Antiplasmin

Abbreviation : α2-AP

Uniprot ID : Q61247

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 1.875 ng/ml

Detection Range : 3.125 – 200 ng/ml

Manual & Datasheet

More information about this Mouse α2-AP (α2-Antiplasmin) ELISA Kit from Elabscience