Monkey PTH (Parathyroid Hormone) ELISA Kit – E-EL-MK0697

Monkey PTH (Parathyroid Hormone) ELISA Kit

The PTH ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PTH. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for PTH and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PTH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of PTH. You can calculate the concentration of PTH in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Parathyroid Hormone

Abbreviation : PTH

Uniprot ID : Q9XT35

Format : 96-well plate

Reactivity : Monkey

ELISA configuration : Sandwich

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 – 1000 pg/ml

Manual & Datasheet

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