Monkey PGF (Placental Growth Factor) ELISA Kit – E-EL-MK0082

Monkey PGF (Placental Growth Factor) ELISA Kit

The PGF ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey PGF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Monkey PGF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey PGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey PGF. You can calculate the concentration of Monkey PGF in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Placental Growth Factor

Abbreviation : PGF

Uniprot ID :

Format : 96-well plate

Reactivity : Monkey

ELISA configuration : Sandwich

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 – 1000 pg/ml

Manual & Datasheet

More information about Monkey PGF (Placental Growth Factor) ELISA Kit from Elabscience