Monkey MCSF (Macrophage Colony Stimulating Factor 1) ELISA Kit – E-EL-MK1669

Monkey MCSF (Macrophage Colony Stimulating Factor 1) ELISA Kit

The MCSF ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey MCSF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Monkey MCSF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey MCSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey MCSF. You can calculate the concentration of Monkey MCSF in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Macrophage Colony Stimulating Factor 1

Abbreviation : MCSF

Uniprot ID : A0A0D9S6I0

Format : 96-well plate

Reactivity : Monkey

ELISA configuration : Sandwich

Sensitivity : 18.75 pg/ml

Detection Range : 31.25 – 2000 pg/ml

Manual & Datasheet

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