Monkey GFAP (Glial Fibrillary Acidic Protein) ELISA Kit – E-EL-MK1769

Monkey GFAP (Glial Fibrillary Acidic Protein) ELISA Kit

The GFAP ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey GFAP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Monkey GFAP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey GFAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey GFAP. You can calculate the concentration of Monkey GFAP in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Glial Fibrillary Acidic Protein

Abbreviation : GFAP

Uniprot ID : Q5ISR6

Format : 96-well plate

Reactivity : Monkey

ELISA configuration : Sandwich

Sensitivity : 0.375 ng/ml

Detection Range : 0.625 – 40 ng/ml

Manual & Datasheet

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