Monkey FGF19 (Fibroblast Growth Factor 19) ELISA Kit – E-EL-MK2213

Monkey FGF19 (Fibroblast Growth Factor 19) ELISA Kit

The FGF19 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FGF19. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for FGF19 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FGF19, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of FGF19. You can calculate the concentration of FGF19 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Fibroblast Growth Factor 19

Abbreviation : FGF19

Uniprot ID : A0A0D9RAA1

Format : 96-well plate

Reactivity : Monkey

ELISA configuration : Sandwich

Sensitivity : 4.688 pg/ml

Detection Range : 7.813 – 500 pg/ml

Manual & Datasheet

More information about Monkey FGF19 (Fibroblast Growth Factor 19) ELISA Kit from Elabscience