Monkey Fcγ (Fc Fragment of IgG) ELISA Kit – E-EL-MK0410

Monkey Fcγ (Fc Fragment of IgG) ELISA Kit

The Fcγ ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey Fcγ. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Monkey Fcγ and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey Fcγ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey Fcγ. You can calculate the concentration of Monkey Fcγ in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Fc Fragment of IgG

Abbreviation : Fcγ

Uniprot ID :

Format : 96-well plate

Reactivity : Monkey

ELISA configuration : Sandwich

Sensitivity : 0.188 ng/ml

Detection Range : 0.313 – 20 ng/ml

Manual & Datasheet

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