Human VF (Visfatin) ELISA Kit – E-EL-H1763

Human VF (Visfatin) ELISA Kit

The Human VF ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for VF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of VF. You can calculate the concentration of VF in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Visfatin

Abbreviation : VF

Uniprot ID : P43490

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.188 ng/ml

Detection Range : 0.313 – 20 ng/ml

Manual & Datasheet

More information about this Human VF (Visfatin) ELISA Kit from Elabscience