Human VEGF165 (Vascular Endothelial Growth Factor165) ELISA Kit – E-EL-H2158

Human VEGF165 (Vascular Endothelial Growth Factor165) ELISA Kit

The Human VEGF165 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VEGF165. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for VEGF165 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VEGF165, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of VEGF165. You can calculate the concentration of VEGF165 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Vascular Endothelial Growth Factor165

Abbreviation : VEGF165

Uniprot ID :

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 18.75 pg/ml

Detection Range : 31.25 – 2000 pg/ml

Manual & Datasheet

More information about this Human VEGF165 (Vascular Endothelial Growth Factor165) ELISA Kit from Elabscience