Human VCL (Vinculin) ELISA Kit – E-EL-H1795

Human VCL (Vinculin) ELISA Kit

The Human VCL ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VCL. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for VCL and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VCL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of VCL. You can calculate the concentration of VCL in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Vinculin

Abbreviation : VCL

Uniprot ID : P18206

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 46.875 pg/ml

Detection Range : 78.125 – 5000 pg/ml

Manual & Datasheet

More information about this Human VCL (Vinculin) ELISA Kit from Elabscience