Human UPP2 (Uridine Phosphorylase 2) ELISA Kit – E-EL-H1513

Human UPP2 (Uridine Phosphorylase 2) ELISA Kit

The Human UPP2 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to UPP2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for UPP2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain UPP2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of UPP2. You can calculate the concentration of UPP2 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Uridine Phosphorylase 2

Abbreviation : UPP2

Uniprot ID : O95045

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 18.75 pg/ml

Detection Range : 31.25 – 2000 pg/ml

Manual & Datasheet

More information about this Human UPP2 (Uridine Phosphorylase 2) ELISA Kit from Elabscience