Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit – E-EL-H1661

Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit

The Human UCP-1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to UCP-1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for UCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain UCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of UCP-1. You can calculate the concentration of UCP-1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Uncoupling Protein 1, Mitochondrial

Abbreviation : UCP-1

Uniprot ID : P25874

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

More information about this Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit from Elabscience