Human UBE1L (Ubiquitin Activating Enzyme E1 Like Protein) ELISA Kit – E-EL-H1255

Human UBE1L (Ubiquitin Activating Enzyme E1 Like Protein) ELISA Kit

The Human UBE1L ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to UBE1L. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for UBE1L and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain UBE1L, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of UBE1L. You can calculate the concentration of UBE1L in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Ubiquitin Activating Enzyme E1 Like Protein

Abbreviation : UBE1L

Uniprot ID : P41226

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.938mU/mL

Detection Range : 1.563 – 100mU/mL

Manual & Datasheet

More information about this Human UBE1L (Ubiquitin Activating Enzyme E1 Like Protein) ELISA Kit from Elabscience