Human SEMA4C (Semaphorin 4C) ELISA Kit – E-EL-H2184

Human SEMA4C (Semaphorin 4C) ELISA Kit

The Human SEMA4C ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SEMA4C. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human SEMA4C and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SEMA4C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SEMA4C. You can calculate the concentration of Human SEMA4C in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Semaphorin 4C

Abbreviation : SEMA4C

Uniprot ID : Q9C0C4

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

More information about this Human SEMA4C (Semaphorin 4C) ELISA Kit from Elabscience