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Human HPA (Heparanase) ELISA Kit – E-EL-H2365

96 rx

797.00$ CAD

0 in stock (expect 7-14 days for delivery)

0 in stock (expect 7-14 days for delivery)

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SKU: E-EL-H2365 Category: Tags: , Brand:

Human HPA (Heparanase) ELISA Kit

The Human HPA ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HPA. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human HPA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HPA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HPA. You can calculate the concentration of Human HPA in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Heparanase

Abbreviation : HPA

Uniprot ID : Q9Y251

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

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96 rx

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