Human ES-Ab (Anti-Endostatin Antibody) ELISA Kit – E-EL-H2167

Human ES-Ab (Anti-Endostatin Antibody) ELISA Kit

The Human ES-Ab ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antigen specific to Human ES-Ab. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antigen. Then a biotinylated detection antigen specific for Human ES-Ab and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ES-Ab, biotinylated detection antigen and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ES-Ab. You can calculate the concentration of Human ES-Ab in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Anti-Endostatin

Abbreviation : ES-Ab

Uniprot ID :

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.938 ng/ml

Detection Range : 1.563 – 100 ng/ml

Manual & Datasheet

More information about this Human ES-Ab (Anti-Endostatin Antibody) ELISA Kit from Elabscience