Human BCL2 / Bcl-2 Protein (His tag) – PKSH031777

Recombinant Human BCL2 / Bcl-2 Protein (His tag)

BCL2 (B-cell leukemia/lymphoma 2, N-Histidine-tagged), also known as Bcl-2, belongs to the Bcl-2 family. Bcl-2 family proteins regulate and contribute to programmed cell death or apoptosis. It is a large protein family and all members contain at least one of four BH (bcl-2 homology) domains. Certain members such as Bcl-2, Bcl-xl and Mcl1 are anti-apoptotic, whilst others are pro-apoptotic. Most Bcl-2 family members contain a C-terminal transmembrane domain that functions to target these proteins to the outer mitochondrial and other intracellular membranes. It is expressed in a variety of tissues. BCL2 blocks the apoptotic death of some cells such as lymphocytes. It also regulates cell death by controlling the mitochondrial membrane permeability and inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.

Synonym(s) : Bcl2 , zBlp2

Species(s) : Human

Protein Expression Host : E. coli

Protein Construction : A DNA sequence encoding the human BCL2 isoform 1 (P10415-) (Met 1-Asp 211) was expressed, with a polyhistide tag at the C-terminus.

Predicted N-terminal amino acid : Met 1

Molecular weight/mass : The recombinant human BCL2 consisting of 221 amino acids and has a calculated molecular mass of 24.7 kDa. It migrates as an approximately 32 KDa band in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the human BCL2 isoform 1 (P10415-) (Met 1-Asp 211) was expressed, with a polyhistide tag at the C-terminus.

Formulation : Lyophilized from sterile 50mM Tris, 20% glycerol, 100mM Arg, pH 8.5

Purity : > 90 % as determined by SDS-PAGE

Endotoxin level :

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet