Human BAFFR (B Cell Activating Factor Receptor) ELISA Kit
The Human BAFFR ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BAFFR. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for BAFFR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BAFFR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of BAFFR. You can calculate the concentration of BAFFR in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: B Cell Activating Factor Receptor
Abbreviation : BAFFR
Uniprot ID : Q96RJ3
Format : 96 rx
Reactivity : Human
ELISA configuration : Sandwich
Sensitivity : 0.094 ng/ml
Detection Range : 0.156 – 10 ng/ml
Additional information
Format | 96 rx |
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