Human ARRβ1 (Arrestin Beta 1) ELISA Kit – E-EL-H0502

Human ARRβ1 (Arrestin Beta 1) ELISA Kit

The Human ARRβ1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ARRβ1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ARRβ1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ARRβ1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ARRβ1. You can calculate the concentration of ARRβ1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Arrestin Beta 1

Abbreviation : ARRβ1

Uniprot ID : P49407

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 37.5 pg/ml

Detection Range : 62.5 – 4000 pg/ml

Manual & Datasheet

More information about this Human ARRβ1 (Arrestin Beta 1) ELISA Kit from Elabscience