Human ApoA1 (Apolipoprotein A1) ELISA Kit – E-EL-H0125

Human ApoA1 (Apolipoprotein A1) ELISA Kit

The Human ApoA1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ApoA1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ApoA1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ApoA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ApoA1. You can calculate the concentration of ApoA1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Apolipoprotein A1

Abbreviation : ApoA1

Uniprot ID : P02647

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 1.875 ng/ml

Detection Range : 3.125 – 200 ng/ml

Manual & Datasheet

More information about this Human ApoA1 (Apolipoprotein A1) ELISA Kit from Elabscience