Human AP36 (Apelin 36) ELISA Kit
The Human AP36 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AP36. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for AP36 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AP36, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of AP36. You can calculate the concentration of AP36 in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Apelin 36
Abbreviation : AP36
Format : 96 rx
Reactivity : Human
ELISA configuration : Competitive
Sensitivity : 28.125 pg/ml
Detection Range : 46.875 – 3000 pg/ml
More information about this Human AP36 (Apelin 36) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
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