Human AP (Aprotinin) ELISA Kit – E-EL-H0484

Human AP (Aprotinin) ELISA Kit

The Human AP ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for AP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of AP. You can calculate the concentration of AP in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Aprotinin

Abbreviation : AP

Uniprot ID : P46937

Format : 96 rx

Reactivity : Human

ELISA configuration : Competitive

Sensitivity : 0.938 ng/ml

Detection Range : 1.563 – 100 ng/ml

Manual & Datasheet

More information about this Human AP (Aprotinin) ELISA Kit from Elabscience