Human Anti-GM1 (Anti-Ganglioside M1 Antibody) ELISA Kit
The Human Anti-GM1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Anti-GM1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Anti-GM1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Anti-GM1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Anti-GM1. You can calculate the concentration of Anti-GM1 in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Anti-Ganglioside M1
Abbreviation : GM1-Ab
Format : 96 rx
Reactivity : Human
ELISA configuration : Sandwich
Sensitivity : 0.938mU/mL
Detection Range : 1.563 – 100mU/mL
More information about this Human Anti-GM1 (Anti-Ganglioside M1 Antibody) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
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