Human ANKRD12 (Ankyrin Repeat Domain-containing Protein 12) ELISA Kit – E-EL-H5648

Human ANKRD12 (Ankyrin Repeat Domain-containing Protein 12) ELISA Kit

The ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ANKRD12. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human ANKRD12 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANKRD12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ANKRD12. You can calculate the concentration of Human ANKRD12 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Ankyrin Repeat Domain-containing Protein 12

Abbreviation : ANKRD12

Uniprot ID : Q6UB98

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

More information about this Human ANKRD12 (Ankyrin Repeat Domain-containing Protein 12) ELISA Kit from Elabscience