Human ALDOA (Aldolase A, Fructose Bisphosphate) ELISA Kit – E-EL-H0309

Human ALDOA (Aldolase A, Fructose Bisphosphate) ELISA Kit

The Human ALDOA ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ALDOA. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ALDOA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ALDOA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ALDOA. You can calculate the concentration of ALDOA in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Aldolase A, Fructose Bisphosphate

Abbreviation : ALDOA

Uniprot ID : P04075

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 37.5 pg/ml

Detection Range : 62.5 – 4000 pg/ml

Manual & Datasheet

More information about this Human ALDOA (Aldolase A, Fructose Bisphosphate) ELISA Kit from Elabscience