Human AFGF/FGF1 (Acidic Fibroblast Growth Factor 1) CLIA Kit – E-CL-H0071

Human AFGF/FGF1 (Acidic Fibroblast Growth Factor 1) CLIA Kit

The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AFGF/FGF1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the;specific antibody. Then a biotinylated detection antibody specific for Human AFGF/FGF1 and Avidin-Horseradish;Peroxidase (HRP) conjugate is added to each micro plate well successively and;incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AFGF/FGF1, biotinylated detection;antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence;immunoassay analyzer. The RLU value is positively associated with the;concentration of Human AFGF/FGF1. You can calculate the;concentration of Human AFGF/FGF1 in the samples by;comparing the RLU value of the samples to the standard curve.

Detected Target: Human AFGF/FGF1

Alternate names: FGF-1, ECGF, ECGF-beta, A-FGF, FGFA, ECGFA, ECGFB, FGF-Alpha, HBGF1, HBGF-1, ECGFB, GLIO703, Heparin-binding growth factor 1

Format : 96 rx

Reactivity : Human AFGF/FGF1

Assay configuration : Sandwich

Sensitivity : 7.5 pg/ml

Detection Range : 12.5 to 800 pg/ml

Manual & Datasheet

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