VB12 (Vitamin B12) ELISA Kit – E-EL-0010

VB12 (Vitamin B12) ELISA Kit

The VB12 ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with VB12. During the reaction, Universal VB12 in the sample or standard competes with a fixed amount of VB12 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal VB12. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal VB12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: VB12

Abbreviation : Vitamin B12, vitamin B-12, cobalamin

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 0,469

Detection Range : 0.781-50 ng/mL

More information about this VB12 (Vitamin B12) ELISA Kit from Elabscience