Human KNG1 (Kininogen 1) ELISA Kit – E-EL-H2255

Human KNG1 (Kininogen 1) ELISA Kit

The Human KNG1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to KNG1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for KNG1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain KNG1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of KNG1. You can calculate the concentration of KNG1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Kininogen 1

Abbreviation : KNG1

Uniprot ID : P01042

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 4.688 pg/ml

Detection Range : 7.813 – 500 pg/ml

Manual & Datasheet

More information about this Human KNG1 (Kininogen 1) ELISA Kit from Elabscience