Human ARHGDIα (Rho GDP Dissociation Inhibitor Alpha ) ELISA Kit – E-EL-H0501

Human ARHGDIα (Rho GDP Dissociation Inhibitor Alpha ) ELISA Kit

The Human ARHGDIα ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ARHGDIα. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ARHGDIα and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ARHGDIα, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ARHGDIα. You can calculate the concentration of ARHGDIα in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rho GDP Dissociation Inhibitor Alpha

Abbreviation : ARHGDIα

Uniprot ID : P52565

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

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