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Which E.Coli Competent Cells to use for Protein Expression ? How much should you pay?

Expression Competent Cell comparison & selection chart

Do you agree that expression competent cells from other providers are too expensive?

To facilitate your expression competent cell selection and save you time and money, Civic Bioscience has build a comparison chart with top vending E.Coli protein expression competent cells used in which you can perform plasmid DNA transformation. Expression competent cells from competitors and from Transgen Biotech (the largest competent cell manufacturer in the world) are compared to make your choice easier. Civic Bioscience provides high-efficiency, low price, chemically competent cells from Transgen Biotech. Dare to compare and be surprised on how much you can save on your competent cells used in your protein expression experiments!

Link to Cloning Competent Cell Selection Chart

BL21 Chemically Competent Cell

CD901-02
126$/ml (10 x 100ul)
  • Transfo. efficiency: > 1 x 107 cfu/ug
  • Genotype: E.Coli B strain F- omp T hsdS(rB- mB-) gal dcm araB::T7 RNAP-tetA
  • Shipping fee: 0$
Buy Now

BL21-AI™ One Shot® Chem. Competent E. coli

C6070-03
777$/ml (20 x 50 ul)
  • Transfo. efficiency: > 1 x 108 cfu/ug
  • Genotype: F- omp T hsdS(rB- mB-) gal dcm araB::T7 RNAP-tetA
  • Shipping fee: not clear, may depend on your location.
Thermo' website

BL21 Competent E.coli

C2530H
231$/ml (20 x 0,05 ml)
  • Transfo. efficiency: > 1 x 107 cfu/ug
  • Genotype : E.Coli B strain fhuA2 [lon] ompT gal [dcm] ΔhsdS
  • Shipping fee : 0$ if ordered online
UofT Medstore pricing

BL21(DE3) Chemically Competent Cell

CD601-02
126$/ml (10 x 100ul)
  • Transfo. efficiency: > 1 x 107 cfu/ug
  • Genotype: E.Coli B strain F- ompT hsdS(rB- mB-) gal dcm(DE3)
  • Shipping fee: 0$
Buy Now

One Shot® BL21(DE3) Chemically Competent E. coli

C6000-03
624$/ml (20 x 50 ul)
  • Transfo. efficiency: > 1 x 108 cfu/ug
  • Genotype: E.Coli B strain F- ompT hsdS(rB- mB-) gal dcm(DE3)
  • Shipping fee: not clear, may depend on your location.
UofT Medstore pricing

BL21(DE3) Competent E.coli

C2527H
231$/ml (20 x 0,05 ml)
  • Transfo. efficiency: > 1 x 107 cfu/ug
  • Genotype : E.Coli B strain fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS
  • Shipping fee : 0$ if ordered online
UofT Medstore pricing

BL21(DE3)pLysS Chemically Competent Cell

CD701-02
126$/ml (10 x 100ul)
  • Transfo. efficiency: > 1 x 107 cfu/ug
  • Genotype: E.Coli B strain F- ompT hsdS(rB- mB-) gal dcm (DE3) pLysS Camr
  • Shipping fee: 0$
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One Shot® BL21(DE3)pLysS Chem. Competent E. coli

C6060-03
749$/ml (20 x 50 ul)
  • Transfo. efficiency: > 1 x 108 cfu/ug
  • Genotype: E.Coli B strain F- ompT hsdS(rB- mB-) gal dcm (DE3) pLysS Camr
  • Shipping fee: not clear, may depend on your location.
Thermo's website

BL21(DE3)pLysS Competent Cells

L1195
250$/ml (20 x 0,05 ml)
  • Transfo. efficiency: > 1 x 107 cfu/ug
  • Genotype : E.Coli B strain F- ompT hsdS(rB- mB-) gal dcm (DE3) pLysS Camr
  • Shipping fee : unknown, may depend on your location
UofT Medstore pricing

Transetta(DE3) Chemically Competent Cell

CD801-02
140$/ml (10 x 100ul)
  • Transfo. efficiency: > 1 x 107 cfu/ug
  • Genotype: E.Coli B strain F- ompT hsdSB(rB- mB-) gal dcm lacY1 (DE3) pRARE(argU, argW, ileX, glyT, leuW, proL)(Camr)
  • Shipping fee: 0$
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Rosetta™(DE3) Competent Cells

70954-4
256$/ml (1 ml)
  • Transfo. efficiency: > 1 x 107 cfu/ug
  • Genotype: E.Coli B strain F- ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE (CamR)
  • Shipping fee: not clear, may depend on your location.
EMD Millipore's website

TransB(DE3) Chemically Competent Cell

CD811-02
140$/ml (10 x 100ul)
  • Transfo. efficiency: > 1 x 107 cfu/ug
  • Genotype: E.Coli B strain F- ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB (KanR,TetR)
  • Shipping fee: 0$
Buy Now

Origami™ B(DE3) Competent Cells

70837-4
245$/ml (1 ml)
  • Transfo. efficiency: > 1 x 107 cfu/ug
  • Genotype: E.Coli B strain F- ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC (DE3) gor522:: Tn10 trxB (KanR, TetR)
  • Shipping fee: not clear, may depend on your location.
EMD Millipore's website

SHuffle T7 Express Competent E.coli

C3029H
280$/ml (6 x 0.05 ml)
  • Transfo. efficiency: > 1 x 107 cfu/ug
  • Genotype: fhuA2 lacZ::T7 gene1 [lon] ompT ahpC gal λatt::pNEB3-r1-cDsbC (SpecR, lacIq) ΔtrxB sulA11 R(mcr-73::miniTn10-TetS)2 [dcm] R(zgb-210::Tn10 -TetS) endA1 Δgor ∆(mcrC-mrr)114::IS10
  • Note: C3029H sold as 300ul for 84$
  • Shipping fee: 0$ if ordered online
EMD Millipore's website

* Transgen Biotech competent cells are distributed by Civic Bioscience Limitée in Canada.

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