STR analysis and Prenatal Sex Genotyping by PCR of Fetal Cell-Free DNA purified from 18 ul of a 10-week pregnant woman's blood PCR Success Story #24 Last Modified on : February 5, 2020 Introduction STR & Sex Analysis cffDNA and gDNA STR & Sex PCR Hifi vs Taq...
A better multiplex PCR protocol for Dusp4 Knockout mice genotyping PCR Success Story #23 by Simon Roy, Ph.D. Published September 27th, 2018 Genetic Info Difficulty or Challenge Optimization Success Protocol Results Conclusion Files Project Description Create a better...
Site-Directed Mutagenesis on a Minicircle using Overlap Extension PCR PCR Success Story #22 Vector Preparation PCR steps Assembly Required Cloning Transformation Validation Project Description Generate double mutants in a minicircle vector with contains very few...
GC-Rich Cloning of HLA Nonclassical Class I Heavy Chain PCR Success Story #21 Project Description Story #21: GC-Rich Cloning HLA Nonclassical Class I Heavy Chain (paid service). A Montreal researcher contacted us to perform the cloning of a Human Leukocyte Antigen...
AT-Rich Vector Amplification by Fast & Steep PCR for Deletion by Site-Directed Mutagenesis PCR Success Story #20 AT-Rich Vector Amplification by Fast & Steep PCR During an early 2017 customer visit in Quebec city, we met with a researcher who was unsuccessful at...
Insertion of Stop Codons by Fast & Steep PCR Site-Directed Mutagenesis and Seamless Assembly PCR Success Story #19 Project Description Stop Codon Insertion by Fast & Steep PCR Site-Directed Mutagenesis and Seamless Assembly (paid service). The researcher located...
16s rRNA gene identification from 3 environmental samples @ Private company PCR Success Story #18 Project Description 16s rRNA gene identification and quantification of bacterial diversity from 3 environmental bacterial populations maintained in industrial bioreactors...
PCR Success Story #17: High-Fidelity PCR Amplification of repetitive sequences and PCR Overlap Assembly of 3 Fragments PCR Success Story #17 Project Description High-Fidelity PCR Amplification of repetitive sequences and PCR Overlap Assembly of 3 Fragments This...
Site-Directed Mutagenesis by PCR Overlap and Fast Mutagenesis Techniques PCR Success Story #16 Project Description Site-Directed Mutagenesis by PCR Overlap and Fast Mutagenesis (paid service) Our work consisted in performing Custom Fast Mutagenesis : design of...
Complete Design, Cloning and Truncation Analysis of a Human Promoter PCR Success Story #15 Project Description Complete Design, Cloning and Truncation Analysis of a Human Promoter (paid service) To protect the research and privacy of the customer who paid for our...
High-Fidelity PCR Amplification of Pseudomonas aeruginosa murC and murE PCR Success Story #14 Project Description High-Fidelity PCR Amplification of Pseudomonas aeruginosa murC and murE This cloning project originated from the lab of a new researcher located at the...
Genotyping by PCR of a Large Colony of Mice PCR Success Story #13 Project Description Mouse Genotyping by PCR of a large mice colony Mouse Genotyping by PCR works well in their lab, but costs 1000$ for 1000 units of Titanium Taq 🙁 This project illustrates one of the...
Base Deletion by Site-Directed Mutagenesis with Extreme GC Content PCR Success Story #12 Project Description Small 1 base deletion from an Extreme GC Content template (up to 94% GC-rich) This project originated from previous exchanges with a customer having problems...
Insertion of a 10xHis Tag by Site-Directed Mutagenesis PCR Success Story #11 Project Description Large 10xHis Insertion by Site-Directed Mutagenesis This project originated from a customer asking us advice on the experimental design for accomplishing an Insertion by...
MC4R Mouse Genotyping by PCR PCR Success Story #10 Project Description KO and KI MC4R Mouse Genotyping by PCR This challenge is actually quite common to labs who have big mice colonies and have to perform a lot of genotyping by PCR each week. This very well known...
High-Fidelity and Specific PCR from human gDNA PCR Success Story #9 Project Description Specific PCR with High-Fidelity DNA Polymerases This project was quite important to solve. We met with a researcher located at the Centre de Recherche du CHU Ste-Justine who had...
Human gDNA Quality Control PCR Success Story #8 Project Description Human gDNA Quality Control The lab cannot perform Human gDNA Quality Control : forced setup of PCR reactions under a hood… This challenge was quite unusual, yet very inconvenient for the...
Tph2 Mouse Genotyping by PCR PCR Success Story #7 Project Description This project consisted in optimizing Tph2 Mouse Genotyping by PCR protocol for a well established lab located at the University of Ottawa. The customer had difficulties genotyping...
Cre and A2A Mouse Genotyping PCR Success Story #6 Project Description Re-design of Cre and A2A Mouse Genotyping This challenge consisted re-designing the Cre and A2A Mouse Genotyping by PCR protocol for a well established lab located at the Centre de Recherche du...
Human CHD2 (long isoform) Difficult PCR Amplification PCR Success Story #5 Project Description Difficult PCR Amplification of CHD2 (long isoform) from human cDNA This project looked pretty simple but it ended up being a HUGE challenge and very Difficult PCR to...
