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Fast MultiSite Mutagenesis System

Up to six mutated PCR fragments are seamlessly assembled by homologous recombination using proprietary mix.

Includes:

Cloning Strategy

  1. Perform PCR reactions sing mutagenic primers
  2. Treat PCR products with DMT enzyme and purify
  3. Mix with the assembly mix
  4. Transform into DMT Competent Cells

Primer Design

Design PCR primers (25-40bp) with 15-25bp overlapping regions

If mutagenic primers are

  • F1 and R1
  • F2 and R2
  • F3 and R3

Then,

  • F2 primer overlaps with R1 primer
  • F3 with R2
  • F1 with R3