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Cloning Vectors and Kits

Cloning Vectors and Kits shop page

pEASY®-Uni Seamless Cloning and Assembly Kit

Achieve directional cloning of up to 5 PCR fragments using homologous recombination.

Assembly Workflow

  1. Prepare vector by PCR or restriction enzyme digestion
  2. Order DNA fragments or prepare by PCR
  3. Mix
  4. Transform

Primer Design

Design PCR primers (25-40bp) with 15-25bp overlapping regions

If fragment primers are

  • F1 and R1
  • F2 and R2
  • F3 and R3

Then,

  • F2 primer overlaps with R1 primer
  • F3 with R2
  • F1 with R3