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pEASY®-Uni Seamless Cloning and Assembly Kit

Achieve directional cloning of up to 5 PCR fragments using homologous recombination.

Fast cloning: 15 minutes
Easy: no restriction enzymes needed
High efficiency: up to 95% positive clones
Seamless: no extra sequences introduced
Includes Trans1-T1 Phage Chemically Competent Cell.

Assembly Workflow

Prepare vector by PCR or restriction enzyme digestion
Order DNA fragments or prepare by PCR
Mix
Transform

Primer Design

Design PCR primers (25-40bp) with 15-25bp overlapping regions

If fragment primers are

F1 and R1
F2 and R2
F3 and R3

Then,

F2 primer overlaps with R1 primer
F3 with R2
F1 with R3

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