Workflow de clonage d'ADN Trucs de PCR et trucs de clonage dMADN par Simon Roy, Ph.D Cette page sera mise Ă jour plusieurs fois. Il vous serait utile de la consulter dans le futur afin d’obtenir des mises Ă jour sur mes protocoles. ”Bookmark” ...
STR analysis and Prenatal Sex Genotyping by PCR of Fetal Cell-Free DNA purified from 18 ul of a 10-week pregnant woman's blood PCR Success Story #24 Last Modified on : February 5, 2020 Introduction STR & Sex Analysis cffDNA and gDNA STR & Sex PCR Hifi vs Taq...
A better multiplex PCR protocol for Dusp4 Knockout mice genotyping PCR Success Story #23 by Simon Roy, Ph.D. Published September 27th, 2018 Genetic Info Difficulty or Challenge Optimization Success Protocol Results Conclusion Files Project Description Create a better...
Site-Directed Mutagenesis on a Minicircle using Overlap Extension PCR PCR Success Story #22 Vector Preparation PCR steps Assembly Required Cloning Transformation Validation Project Description Generate double mutants in a minicircle vector with contains very few...
Overlap Extension PCR Protocol PCR Protocol for DNA assembly by PCR extension of overlapping DNA fragments. PCR overlap extension is useful for DNA cloning and site-directed mutagenesis. Here, you will find 2 different protocols a standard protocol for performing...
Fast & Steep PCR Protocol Fast PCR Protocol for Cloning and Site-Directed Mutagenesis Fast & Steep PCR can be used for quick DNA amplification from plasmid DNA, purified DNA fragments and assembly PCR by extension of overlapping DNA fragments. Do it with FLYMain...
Fast Mutagenesis Protocol with FM111 Site-Directed Mutagenesis using WVA and partially overlapping primers Get the Fast Mutagenesis KitGo to the Main SDM page ; TransBionovo's Fast Mutagenesis Protocol Advantages Semi-exponential amplification PCR. Both primers...
QuickChange Site-Directed Mutagenesis Protocol Site-Directed Mutagenesis using WVA and complementary primers Go to the Main SDM pageGet inspired by our PCR Success Stories ; QuickChange Site-Directed Mutagenesis Advantages Both primers contain the mutation. In vitro...
KLD Site-Directed Mutagenesis Protocol Site-Directed Mutagenesis using WVA, back-to-back primers and KLD Build your own KLD Mutagenesis KitGo to the Main SDM Page ; KLD Site-Directed Mutagenesis Protocol using Back-to-Back Primers Advantages of KLD Site-Directed...
GC-Rich Cloning of HLA Nonclassical Class I Heavy Chain PCR Success Story #21 Project Description Story #21: GC-Rich Cloning HLA Nonclassical Class I Heavy Chain (paid service). A Montreal researcher contacted us to perform the cloning of a Human Leukocyte Antigen...
AT-Rich Vector Amplification by Fast & Steep PCR for Deletion by Site-Directed Mutagenesis PCR Success Story #20 AT-Rich Vector Amplification by Fast & Steep PCR During an early 2017 customer visit in Quebec city, we met with a researcher who was unsuccessful at...
Insertion of Stop Codons by Fast & Steep PCR Site-Directed Mutagenesis and Seamless Assembly PCR Success Story #19 Project Description Stop Codon Insertion by Fast & Steep PCR Site-Directed Mutagenesis and Seamless Assembly (paid service). The researcher located...
16s rRNA gene identification from 3 environmental samples @ Private company PCR Success Story #18 Project Description 16s rRNA gene identification and quantification of bacterial diversity from 3 environmental bacterial populations maintained in industrial bioreactors...
PCR Success Story #17: High-Fidelity PCR Amplification of repetitive sequences and PCR Overlap Assembly of 3 Fragments PCR Success Story #17 Project Description High-Fidelity PCR Amplification of repetitive sequences and PCR Overlap Assembly of 3 Fragments This...
Site-Directed Mutagenesis by PCR Overlap and Fast Mutagenesis Techniques PCR Success Story #16 Project Description Site-Directed Mutagenesis by PCR Overlap and Fast Mutagenesis (paid service) Our work consisted in performing Custom Fast Mutagenesis : design of...
Complete Design, Cloning and Truncation Analysis of a Human Promoter PCR Success Story #15 Project Description Complete Design, Cloning and Truncation Analysis of a Human Promoter (paid service) To protect the research and privacy of the customer who paid for our...
High-Fidelity PCR Amplification of Pseudomonas aeruginosa murC and murE PCR Success Story #14 Project Description High-Fidelity PCR Amplification of Pseudomonas aeruginosa murC and murE This cloning project originated from the lab of a new researcher located at the...
Genotyping by PCR of a Large Colony of Mice PCR Success Story #13 Project Description Mouse Genotyping by PCR of a large mice colony Mouse Genotyping by PCR works well in their lab, but costs 1000$ for 1000 units of Titanium Taq đ This project illustrates one of the...
Base Deletion by Site-Directed Mutagenesis with Extreme GC Content PCR Success Story #12 Project Description Small 1 base deletion from an Extreme GC Content template (up to 94% GC-rich) This project originated from previous exchanges with a customer having problems...
Insertion of a 10xHis Tag by Site-Directed Mutagenesis PCR Success Story #11 Project Description Large 10xHis Insertion by Site-Directed Mutagenesis This project originated from a customer asking us advice on the experimental design for accomplishing an Insertion by...