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Overlap Extension PCR Protocol

Overlap Extension PCR Protocol

Overlap Extension PCR Protocol PCR Protocol for DNA assembly by PCR extension of overlapping DNA fragments. PCR overlap extension is useful for DNA cloning and site-directed mutagenesis. Here, you will find 2 different protocols a standard protocol for performing...
Fast Mutagenesis System Protocol

Fast Mutagenesis System Protocol

Fast Mutagenesis Protocol with FM111 Site-Directed Mutagenesis using WVA and partially overlapping primers Get the Fast Mutagenesis KitGo to the Main SDM page ; TransBionovo's Fast Mutagenesis Protocol Advantages Semi-exponential amplification PCR. Both primers...
QuickChange Site-Directed Mutagenesis Protocol

QuickChange Site-Directed Mutagenesis Protocol

QuickChange Site-Directed Mutagenesis Protocol Site-Directed Mutagenesis using WVA and complementary primers Go to the Main SDM pageGet inspired by our PCR Success Stories ; QuickChange Site-Directed Mutagenesis Advantages Both primers contain the mutation. In vitro...
Success #13: Mouse Genotyping by PCR of a Large Colony

Success #13: Mouse Genotyping by PCR of a Large Colony

Genotyping by PCR of a Large Colony of Mice PCR Success Story #13 Project Description Mouse Genotyping by PCR of a large mice colony Mouse Genotyping by PCR works well in their lab, but costs 1000$ for 1000 units of Titanium Taq 🙁 This project illustrates one of the...
Success #10: MC4R Mouse Genotyping by PCR

Success #10: MC4R Mouse Genotyping by PCR

MC4R Mouse Genotyping by PCR PCR Success Story #10 Project Description KO and KI MC4R Mouse Genotyping by PCR This challenge is actually quite common to labs who have big mice colonies and have to perform a lot of genotyping by PCR each week. This very well known...