X-gal – GF201-01

X-gal

X-gal is a substrate of β-galactosidase. It shows a blue color after hydrolyzation. Using this property, when lacZ-deficient cell as a host is transformed with vector DNA carrying lacZ gene, IPTG is added together with X-gal into culture medium, based on the α-complementation of β-galactosidase, recombinant clones can be easily identified upon whether white colonies are observed.

Applications

• Blue / white colony screening

Concentration

1 ml at 20 mg/ml

Storage

at -20°C in dark for six months

Manual and Datasheet

GF201 – X-Gal