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Vimentin Antibody – MCA-2A52

  • Clonality : Mouse Monoclonal
  • Applications : WB | IF/ICC | IHC
  • Reactivity : Human | Rat

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Encor Mouse Monoclonal to Vimentin 

Vimentin is a protein subunit of 10nm or intermediate filaments, which are major components of the cytoskeleton in most cell types. Vimentin is the major intermediate filament subunit found in mesenchymal cells, and was first named and characterized in a collaborative study from the labs of German scientists Werner Franke and Klaus Weber (1). The name derives from the Latin “Vimentum“, meaning arrays of flexible rods such as in lattices, filigrees and wicker-work, which describes the intermediate filament network quite well. Vimentin is also found in many cell types in tissue culture, most notably fibroblasts, and in developing neuronal and astrocytic precursor cells in the central nervous system.
Many cell lines such as HEK293, HeLa, 3T3 and Cos cells contain prominent vimentin networks. Vimentin frequently forms copolymers with other intermediate filament proteins, such as GFAP (in astrocytes, ependymal cells and neural stem cells), with desmin (in muscle and endothelial cells) and neurofilament proteins (in developing neurons). A E151K point mutation in the vimentin gene was shown to be causative of an autosomal dominant pulverulent cataract disease, but so far only in a single patient (2).

Vimentin is a major protein of eye lens and cornea, and this mutation renders the molecule unable assemble into normal 10nm filaments. Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. The immunogen used to generate our antibody was recombinant human vimentin expressed in and purified from E. coli. The same immunogen was used to produce our other monoclonal antibody to vimentin MCA-2D1. We also market a very popular chicken polyclonal antibody to vimentin, CPCA-Vim. The HGNC name for this protein is VIM.


HGNC name(s) : VIM


Host : Mouse


Clonality : Monoclonal


ID : EnCor Biotechnology Vimentin 2A52


Reactivity : Human | Rat


Isotype : IgG1


Conjugation : none


Immunogen : Recombinant full length human


Mass of detected protein : 50 kDa


Uniprot ID : P08670


KGNC name : VIM


RRID # : AB_2572396


Purification : Affinity purified at 1 mg/mL


Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.


Validated applications : WB | IF/ICC | IHC


Suggested Dilutions: 

WB: 1:10 000. ICC/IF and IHC: 1:1 000.


Datasheet


Link to on EnCor’s website 


References :

1. Franke, W. W., Schmid, E., Osborn, M. and Weber, K. Different intermediate-sized filaments distinguished by immunofluorescence microscopy. Proc. Natl. Acad. Sci. USA 75:5034–5038 (1978).

2. Muller, M., Bhattacharya, S. S., Moore, T., Prescott, Q., Wedig, T., Herrmann, H., Magin, T. M. Dominant cataract formation in association with a vimentin assembly disrupting mutation. Hum. Molec. Genet. 18:1052-1057 (2009).


Additional information

Format

50 ul, 100 ul, 500 ul

Supplier

Host

Mouse

Clonality

Monoclonal

Reactivity

Human, Rat

Validated Applications

WB, IHC, IF/ICC

Conjugation

None

Isotype

IgG1

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299.00$1,597.00$ CAD

Clear

Cashback Reward : Earn up to 159$!