VC (Vitamin C) ELISA Kit – E-EL-0011

VC (Vitamin C) ELISA Kit

The VC ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VC. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for VC and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of VC. You can calculate the concentration of VC in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: VC

Abbreviation : Vitamin C, L-ascorbic acid, ascorbate

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 0,938

Detection Range : 1.563-100 μg/ml

More information about this VC (Vitamin C) ELISA Kit from Elabscience