VB6 (Vitamin B6) ELISA Kit – E-EL-0008

VB6 (Vitamin B6) ELISA Kit

The VB6 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VB6. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for VB6 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VB6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of VB6. You can calculate the concentration of VB6 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: VB6

Abbreviation :

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 2,813

Detection Range : 4.688-300 ng/mL

More information about this VB6 (Vitamin B6) ELISA Kit from Elabscience