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Ubiquitin Antibody – RPCA-Ubi

  • Clonality : Rabbit Polyclonal
  • Applications : WB | IF/ICC | IHC
  • Reactivity : Human | Rat | Mouse

$338.00$1,810.00 CAD

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Encor Rabbit polyclonal to Ubiquitin

Ubiquitin is a highly conserved globular 76 amino acid protein of about 8.5 kDa molecular weight. It has a important role in the targeting of proteins for proteolytic degradation. Proteins to be degraded are covalently coupled to the C-terminus of ubiquitin by means of ubiquitin ligases. The ubiquitin itself is frequently also ubiquitinated, producing a polyubiquitin chain. The polyubquitinated complex is then recognized by a complex of degradative enzymes which together form the proteosome. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions seen in serious human disease states which appear to be resistant to normal degradation, so that ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer’s disease, the Lewy bodies seen in Parkinson’s disease, and Pick bodies found in Pick’s disease are all heavily ubiquitinated and can all be readily visualized with ubiquitin antibodies of appropriate specificity. Ubiquitin antibodies have become very widely used for such studies. The HGNC name for this protein is UBB, UBC.


HGNC name(s) : UBB, UBC


Host : Rabbit


Clonality : Polyclonal


ID : EnCor Biotechnology Ubiquitin Ubi


Reactivity : Human | Rat | Mouse


Isotype : IgG


Conjugation : none


Immunogen : Purified bovine


Mass of detected protein : 8.5 kDa


Uniprot ID : P0CG47, P0CG48


KGNC name : UBB, UBC


RRID # :


Purification : Serum


Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.


Validated applications : WB | IF/ICC | IHC


Suggested Dilutions:

WB: 1:5 000-1:10 000. IF/ICC and IHC: 1:500-1:1 000.


Datasheet


Link to on EnCor’s website


References :

Perry, G. et al. Proc. Natl. Acad. Sci. USA 84, 3033-3036 (1987)
Shaw, G. and Chau, V. Proc. Natl. Acad. Sci. USA 85, 2854-2858 (1988)
Hirano, S., et al. Cell 70: 293-301 (1992)
Cuervo, A.M., et al. Mol. Biol. 9: 1995-2010 (1995)
Sternsdorf, T., et al. J. Cell Biol. 139: 1621-1634 (1997)
Tae-Wan Kim, et al. J. Biol. Chem. 272: 11006-11010 (1997)
Verdier, F., et al. J. Cell Biol. 273: 28185-28190 (1998)
Laroia, G., et al. Science 284: 499-502 (1999)
Marti, A., et al. Nature Cell Biol. 1: 14-19 (1999)
Sternsdorf, T., et al. Mol. Cell Biol. 19: 5170-5178 (1999)

Recent Papers using MCA-Ubi-1:

1. Fortun J, Go JC, Li J, Amici SA, Dunn WA Jr, Notterpek L. Alterations in degradative pathways and protein aggregation in a neuropathy model based on PMP22 overexpression. Neurobiol Dis. 22:153-164 2006

2. Boutajangout A, Authelet M, Blanchard V, Touchet N, Tremp G, Pradier L, Brion JP. Characterisation of cytoskeletal abnormalities in mice transgenic for wild-type human tau and familial Alzheimer’s disease mutants of APP and presenilin-1. Neurobiol Dis. 15:47-60 (2004).

3. Wang DS, Bennett DA, Mufson EJ, Mattila P, Cochran E, Dickson DW. Contribution of changes in ubiquitin and myelin basic protein to age-related cognitive decline. Neurosci. Res. 48:93-100 (2004).

4. He CZ, Hays AP. Expression of peripherin in ubiquinated inclusions of amyotrophic lateral sclerosis. J. Neurol. Sci. 217:47-54 (2004).

5. Ungureanu D, Saharinen P, Junttila I, Hilton DJ, Silvennoinen O. Regulation of Jak2 through the ubiquitin-proteasome pathway involves phosphorylation of Jak2 on Y1007 and interaction with SOCS-1. Mol Cell Biol. 22:3316-26 (2002)

6. Wirbelauer C, Sutterluty H, Blondel M, Gstaiger M, Peter M, Reymond F, Krek W. The F-box protein Skp2 is a ubiquitylation target of a Cul1-based core ubiquitin ligase complex: evidence for a role of Cul1 in the suppression of Skp2 expression in quiescent fibroblasts. EMBO J. 19:5362-75 (2000).

7. Harris KF, Shoji I, Cooper EM, Kumar S, Oda H, Howley PM. Ubiquitin-mediated degradation of active Src tyrosine kinase. Proc Natl Acad Sci U S A. 96:13738-43 (1999).

8. Sternsdorf T, Puccetti E, Jensen K, Hoelzer D, Will H, Ottmann OG, Ruthardt M. PIC-1/SUMO-1-modified PML-retinoic acid receptor alpha mediates arsenic trioxide-induced apoptosis in acute promyelocytic leukemia. Mol Cell Biol. 19:5170-8 (1999).

9. Marti A, Wirbelauer C, Scheffner M, Krek W. Interaction between ubiquitin-protein ligase SCFSKP2 and E2F-1 underlies the regulation of E2F-1 degradation. Nat Cell Biol. 1:14-9 (1999).


Additional information

Format

50 ul, 100 ul, 500 ul

Supplier

Host

Rabbit

Clonality

Polyclonal

Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC, IF/ICC

Conjugation

None

Isotype

IgG

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